Figure 2.

L-SIGN and DC-SIGN transfectants bind HCV-E2. L-SIGN (Top), DC-SIGN (Middle), and control HeLa cells (Bottom) were incubated with HCV-E2-coated beads prepared by using a panel of anti-E2 mAbs. Adhesion was quantified by fluorescence-activated cell sorter analysis in the presence of adherence buffer (filled bars) and was blocked by mannan (open bars). Different anti-E2 mAbs are indicated on the x axis, and the y axis represents the percentage of cells that have bound beads. One representative experiment out of three is shown.