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. 2003 Apr 2;100(8):4527–4532. doi: 10.1073/pnas.0431081100

Figure 1.

Figure 1

NM fiber formation. (a Left) NM fibers assembled at a protein concentration of >25 μM with fast rotation, which results in many short fibers (average fiber length from 60 to 200 nm). (Right) NM fibers assembled at a protein concentration of 5 μm with fast rotation, which results in long fibers. Fibers were sonciated as described (32) and both were imaged on mica by AFM. (b) The conditions of a Left led to controlled, reproducible formation of amyloid fibers with defined fiber length as visualized on mica by AFM. At seed:soluble NM ratios of 1:1 (wt/wt), resulting fibers showed an average length of 500 ± 100 nm. Increasing the soluble NM concentration led to increased fiber lengths. At ratios of 1:16 of seed:soluble NM the resulting fibers were ≈5 ± 1 μm long. (c) Natural alignment of NM fibers. (Left) Lower magnification. (Right) Higher magnification (imaged by TEM).