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. 2003 Apr 3;100(8):4533–4538. doi: 10.1073/pnas.0836650100

Figure 1.

Figure 1

Phagolysosome formation by CHO cells expressing WT or mutant forms of FcγRIIA. Cells were preloaded with rhodamine dextran. Cells were incubated with IgG-coated sheep erythrocytes (EA) for 30 min at 37°C then transferred to 4°C. External EA were labeled with fluorescent anti-IgG or removed by hypotonic lysis. Fluorescence microscopy was used to monitor the colocalization of rhodamine dextran with internalized targets. (a and b) Arrowheads indicate areas of colocalization of the internal EA with dextran in WT FcγRIIA cells. (c and d) Mutant FcγRIIA (AAA)-expressing cells; arrowheads point to internal EA that lack colocalization with dextran. (e) Quantitative data concerning the colocalization of EA with fluorescent dextran. In the WT FcγRIIA, ≈90% of targets were colocalized with dextran. However, mutation of the L-T-L motif reduced the percent of targets colocalized with dextran to levels previously obtained with a tailless form of FcγRIIA, suggesting that the L-T-L motif participates in phagolysosome fusion.