Figure 1.

Transactivity of AR hinge deletion mutants and effect of FLNa on AR function. (A) The AR consists of a TAD and a DBD, linked to the LBD by a hinge region. The AR hinge (amino acids 622–670) used as bait in the yeast two-hybrid screen is in bold. WT AR or the deletion mutants [(Δ628–646), (Δ647–670), and (Δ622–670)] were tested for their ability to transactivate the ARE₂-Luc reporter in the presence of 1 nM DHT (WT AR set at 100% ± SEM). (B) Structure of monomeric FLNa. Indicated in this schematic representation are the actin-binding domain (ABD), hinge-1 and -2 (H1, H2), and the 24 Ig-like repeats. (C and D) WT AR was coexpressed with increasing amounts of FLNa, and androgenic activity with (filled bars) or without (open bars) 1 nM DHT was measured with ARE₂-Luc (C) or prostate-specific antigen–luciferase (D). The arrow (C Upper) indicates immunoblot analyses of AR protein from representative cell lysates.