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. 1984 Jul;57(1):123–129.

Interleukin-2 reverses deficient cell-mediated immune responses in rheumatoid arthritis.

P Emery, G S Panayi, A M Nouri
PMCID: PMC1536082  PMID: 6611230

Abstract

The depressed cell-mediated immunity in rheumatoid arthritis was investigated in vivo by cutaneous hypersensitivity responses to seven antigens including tuberculin PPD, and in vitro by lymphocyte transformation to the latter antigen. In vivo 40% of rheumatoid patients were anergic compared to 2% of controls (P less than 0.001) with an associated reduction in sum score (5.9 +/- 6.5 vs 15.3 +/- 8.7, P less than 0.001). In vitro lymphocyte proliferation to PPD was also significantly depressed (P less than 0.001) and could not be reversed by indomethacin. A significant correlation between the in vivo sum scored (induration in mm) and in vitro thymidine incorporation (d/min) (r = 0.59, P less than 0.001) was found. In an attempt to overcome the depressed in vitro response the addition of a crude supernatant from a mixed lymphocyte reaction was found to return the PPD stimulated lymphocyte proliferation to the normal range. This effect was mimicked by purified IL-2 but not purified IL-1. The implications of this finding are are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alcocer-Varela J., Alarcón-Segovia D. Decreased production of and response to interleukin-2 by cultured lymphocytes from patients with systemic lupus erythematosus. J Clin Invest. 1982 Jun;69(6):1388–1392. doi: 10.1172/JCI110579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andersson J., Grönvik K. O., Larsson E. L., Coutinho A. Studies on T lymphocyte activation. I. Requirements for the mitogen-dependent production of T cell growth factors. Eur J Immunol. 1979 Aug;9(8):581–587. doi: 10.1002/eji.1830090802. [DOI] [PubMed] [Google Scholar]
  3. Goodwin J. S., DeHoratius R., Israel H., Peake G. T., Messner R. P. Suppressor cell function in sarcoidosis. Ann Intern Med. 1979 Feb;90(2):169–173. doi: 10.7326/0003-4819-90-2-169. [DOI] [PubMed] [Google Scholar]
  4. Goodwin J. S., Messner R. P., Bankhurst A. D., Peake G. T., Saiki J. H., Williams R. C., Jr Prostaglandin-producing suppressor cells in Hodgkin's disease. N Engl J Med. 1977 Nov 3;297(18):963–968. doi: 10.1056/NEJM197711032971802. [DOI] [PubMed] [Google Scholar]
  5. Haregewoin A., Godal T., Mustafa A. S., Belehu A., Yemaneberhan T. T-cell conditioned media reverse T-cell unresponsiveness in lepromatous leprosy. Nature. 1983 May 26;303(5915):342–344. doi: 10.1038/303342a0. [DOI] [PubMed] [Google Scholar]
  6. Harel-Bellan A., Joskowicz M., Fradelizi D., Eisen H. Modification of T-cell proliferation and interleukin 2 production in mice infected with Trypanosoma cruzi. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3466–3469. doi: 10.1073/pnas.80.11.3466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hopper K. E., Cahill J. M. Immunoregulation by macrophages II. Separation of mouse peritoneal macrophages having tumoricidal and bactericidal activities and those secreting PGE and interleukin I. J Reticuloendothel Soc. 1983 Jun;33(6):443–456. [PubMed] [Google Scholar]
  8. Lockshin M. D., Eisenhauer A. C., Kohn R., Weksler M., Block S., Mushlin S. B. Cell-mediated immunity in rheumatic diseases. II. Mitogen responses in RA, SLE, and other illnesses: correlation with T- and B-lymphocyte populations. Arthritis Rheum. 1975 May-Jun;18(3):245–250. doi: 10.1002/art.1780180308. [DOI] [PubMed] [Google Scholar]
  9. Mason U. G., 3rd, Greenberg L. E., Yen S. S., Kirkpatrick C. H. Indomethacin-responsive mononuclear cell dysfunction in "atypical" mycobacteriosis. Cell Immunol. 1982 Jul 15;71(1):54–65. doi: 10.1016/0008-8749(82)90495-6. [DOI] [PubMed] [Google Scholar]
  10. Miller S. D., Jones H. E. Correlation of lymphocyte transformation with tuberculin skin-test sensitivity. Am Rev Respir Dis. 1973 Apr;107(4):530–538. doi: 10.1164/arrd.1973.107.4.530. [DOI] [PubMed] [Google Scholar]
  11. Paganelli R., Aiuti F., Beverley P. C., Levinsky R. J. Impaired production of interleukins in patients with cell-mediated immunodeficiencies. Clin Exp Immunol. 1983 Feb;51(2):338–344. [PMC free article] [PubMed] [Google Scholar]
  12. Palacios R. Mechanism of T cell activation: role and functional relationship of HLA-DR antigens and interleukins. Immunol Rev. 1982;63:73–110. doi: 10.1111/j.1600-065x.1982.tb00412.x. [DOI] [PubMed] [Google Scholar]
  13. Panayi G. S. Does rheumatoid arthritis have a clinicopathological spectrum similar to that of leprosy? Ann Rheum Dis. 1982 Feb;41(1):102–103. doi: 10.1136/ard.41.1.102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Patel V., Panayi G. S., Shepherd P., Richter M., Harkness J., Gibson T. Lymphocyte studies in rheumatoid arthritis. V. Suppressor cell function in peripheral blood. Scand J Rheumatol. 1982;11(3):133–137. doi: 10.3109/03009748209098178. [DOI] [PubMed] [Google Scholar]
  15. Petersen E. A., Neva F. A., Oster C. N., Bogaert Diaz H. Specific inhibition of lymphocyte-proliferation responses by adherent suppressor cells in diffuse cutaneous leishmaniasis. N Engl J Med. 1982 Feb 18;306(7):387–392. doi: 10.1056/NEJM198202183060702. [DOI] [PubMed] [Google Scholar]
  16. ROPES M. W., BENNETT G. A., COBB S., JACOX R., JESSAR R. A. 1958 Revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis. 1958 Dec;9(4):175–176. [PubMed] [Google Scholar]
  17. Rode H. N., Christou N. V., Bubenik O., Superina R., Gordon J., Meakins J. L., MacLean L. D. Lymphocyte function in anergic patients. Clin Exp Immunol. 1982 Jan;47(1):155–161. [PMC free article] [PubMed] [Google Scholar]
  18. Räsänen L. Skin test reactivity and in vitro responses to microbes and microbial antigens. Clin Exp Immunol. 1980 Jun;40(3):566–572. [PMC free article] [PubMed] [Google Scholar]
  19. Seitz M., Deimann W., Gram N., Hunstein W., Gemsa D. Characterization of blood mononuclear cells of rheumatoid arthritis patients. I. Depressed lymphocyte proliferation and enhanced prostanoid release from monocytes. Clin Immunol Immunopathol. 1982 Dec;25(3):405–416. doi: 10.1016/0090-1229(82)90205-7. [DOI] [PubMed] [Google Scholar]
  20. Silverman H. A., Johnson J. S., Vaughan J. H., McGlamory J. C. Altered lymphocyte reactivity in rheumatoid arthritis. Arthritis Rheum. 1976 May-Jun;19(3):509–515. doi: 10.1002/art.1780190301. [DOI] [PubMed] [Google Scholar]
  21. Turk J. L., Bryceson A. D. Immunological phenomena in leprosy and related diseases. Adv Immunol. 1971;13:209–266. doi: 10.1016/s0065-2776(08)60185-6. [DOI] [PubMed] [Google Scholar]
  22. Watson J., Gillis S., Marbrook J., Mochizuki D., Smith K. A. Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines. J Exp Med. 1979 Oct 1;150(4):849–861. doi: 10.1084/jem.150.4.849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Waxman J., Lockshin M. D., Schnapp J. J., Doneson I. N. Cellular immunity in rheumatic diseases. I. Rheumatoid arthritis. Arthritis Rheum. 1973 Jul-Aug;16(4):499–506. doi: 10.1002/art.1780160410. [DOI] [PubMed] [Google Scholar]
  24. Wolinsky S. I., Goodwin J. S., Messner R. P., Williams R. C., Jr Role of prostaglandin in the depressed cell-mediated immune response in rheumatoid arthritis. Clin Immunol Immunopathol. 1980 Sep;17(1):31–37. doi: 10.1016/0090-1229(80)90070-7. [DOI] [PubMed] [Google Scholar]

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