Table 1.
Respiratory rates and control parameters of isolated rat liver, skeletal muscle, and cardiac muscle mitochondria with either pyruvate, lactate, succinate, or glutamate as substrates in the presence or absence of OX or CINN
| Substrate | Tissue | State 3 | State 4 | RCI | ADP/O |
|---|---|---|---|---|---|
| Pyruvate | Liver | 120.3 ± 7.4 | 46.6 ± 3.7 | 5.2 ± 1.1 | 3.00 ± 0.01 |
| Muscle | 293.4 ± 22.6 | 72.4 ± 5.1 | 12.3 ± 0.9 | 3.04 ± 0.03 | |
| Heart | 168.9 ± 23.3 | 70.2 ± 7.8 | 5.7 ± 0.6 | 3.03 ± 0.01 | |
| Pyruvate + OX | Liver | 177.5 ± 21.5 | 80.1 ± 9.4 | 6.0 ± 0.4 | 3.00 ± 0.01 |
| Muscle | 413.0 ± 41.4 | 76.8 ± 5.4 | 9.7 ± 1.2 | 3.19 ± 0.16 | |
| Heart | 265.8 ± 42.4 | 80.1 ± 9.4 | 4.5 ± 0.5 | 3.25 ± 0.27 | |
| Pyruvate + CINN | Liver | 50.3 ± 3.1 | – | – | – |
| Muscle | 89.2 ± 2.0 | – | – | – | |
| Heart | 41.5 ± 6.0 | – | – | – | |
| Succinate | Liver | 177.5 ± 21.5 | – | 6.0 ± 1.0 | 2.23 ± 0.06 |
| Muscle | – | – | – | – | |
| Heart | – | – | – | – | |
| Pyruvate + CINN + succinate | Liver | 167.5 ± 5.0 | 57.8 ± 4.0 | 5.6 ± 1.0 | |
| Muscle | – | – | – | – | |
| Heart | – | – | – | – | |
| Lactate | Liver | 166.1 ± 17.9 | 66.5 ± 4.8 | 5.6 ± 0.9 | 3.00 ± 0.01 |
| Muscle | 314.4 ± 42.4 | 63.4 ± 11.8 | 21.1 ± 2.0 | 3.01 ± 0.03 | |
| Heart | 142.4 ± 14.7 | 59.3 ± 6.4 | 5.9 ± 0.9 | 3.16 ± 0.27 | |
| Lactate + OX | Liver | 85.2 ± 11.4 | – | – | – |
| Muscle | 38.0 ± 2.6 | – | – | – | |
| Heart | 46.7 ± 12.0 | – | – | – | |
| Lactate + CINN | Liver | 55.6 ± 1.0 | – | – | – |
| Muscle | 41.5 ± 6.0 | – | – | – | |
| Heart | 41.5 ± 6.0 | – | – | – | |
| Lactate + OX + glutamate | Liver | 258.7 ± 16.0 | 87.0 ± 13.0 | 8.7 ± 2.0 | – |
| Muscle | 322.8 ± 27.2 | 103.8 ± 8.6 | 3.5 ± 1.3 | – | |
| Heart | 258.7 ± 16.0 | 87.0 ± 13.0 | 8.9 ± 1.0 | – |
Respiratory rates in nmol O2/mg mitochondrial protein per min. Substrate concentrations: pyruvate and lactate (10 mM + 2.5 mM malate); glutamate (10 mM); succinate (10 mM); OX (50 mM); CINN (5 mM). RCI, Respiratory Control Index.