Table 5.
Comparison of proportions of replication-competent clones containing 184V with dye primer estimates
| Patient | Mos. on HAART at time of virus isolation | Positive wells/total wells at approximate endpoint dilution | Biological clones with 184V/total positive clones (95% confidence interval for %184V) | % 184V by dye primer sequences from PBMC, %* |
|---|---|---|---|---|
| M1 | 58 | 29/70 | 12/29 (28–57%) | 81 ± 22 |
| M2 | 40 | 18/100 | 3/18 (8–36%) | 5 ± 8 |
| M3 | 58 | 5/50 | 2/5 (15–79%) | 40 ± 27 |
| D1 | 51 | 3/24 | 0/3 (0–53%) | 13 ± 14 |
| D2 | 51 | 5/24 | 4/5 (42–94%) | 37 ± 17 |
| D3 | 56 | 0/24 | Undetected | <3 |
With the exception of M1, fractions of culture positive wells indicate >80% likelihood that viral isolates were clonal. 184M and 184V proportions were not statistically different between cocultured isolates of replication-competent virus and estimates from dye primer sequencing of HIV DNA in PBMC.
*
Extrapolated to time of coculture from 184V proportions measured after year 1 on HAART.