Figure 2.

Effects of insect feeding on the accumulation of wound-inducible gene products. Leaves of nontransformed (wt) potatoes and plants from a LOX-H3 antisense line (H3-4) were collected before (0) or at the indicated times (in hours) after long-term infestation with CPB larvae. Total RNA (A) and protein (B) were isolated, and LOX-H1, LOX-H3, and pin2 products were identified by hybridization to the corresponding cDNA probes (Northern blots in A) and antisera (Western blots in B). Equal RNA loading on Northern blots was verified by hybridization with a 18S ribosomal cDNA probe (rRNA). Equal protein loading was verified by visualization of the large subunit of Rubisco (RBCL) stained with Ponceau Red.