Figure 3.

Complementation of the effects of LOX-H3 depletion by exogenous treatment with jasmonic acid. (A) Leaves of nontransformed (WT) and LOX-H3-depleted (H3-4) plants sprayed with a 50 μM JA solution were harvested for total RNA extraction at the times after treatment indicated (in hours) above the lanes. Control leaves were collected before JA treatment (0). (B) Some leaves from the plants used for the experiments shown in A were enclosed in polythene bags before treatment to prevent direct spraying with JA. These systemic leaves were collected at the same time as the locally treated ones, as indicated, and were used for total RNA isolation. Northern blots were hybridized with radioactive cDNA probes as indicated on the left. Equal RNA loading was verified by hybridization with a 18S ribosomal cDNA probe (rRNA).