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PCR analysis of the integration of antisense Pt4CL1 and sense LsCAld5H transgenes in various transgenic aspen (lane numbers represent different transgenic lines). A 1-kb DNA ladder was used for both panels (lane M). The PCR fragments seen are 1.66 and 1.61 kb in size, encompassing a portion of the aspen xylem-specific promoter and the full-length antisense Pt4CL and sense LsCAld5H coding sequences, respectively. Such transgene fragments were absent from the control (lane C).
