Abstract
Peripheral blood lymphocytes from 15 healthy controls and 59 patients with idiopathic glomerulonephritis were studied to determine whether an imbalance exists among human T cell subsets in these diseases. Twenty of the patients studied had a minimal change nephropathy (10 with nephrotic syndrome and 10 in sustained remission); 27 had a membranous glomerulonephritis (12 with nephrotic syndrome, six with isolated proteinuria and nine in complete remission); 12 patients had an IgA glomerulonephritis with heamaturia and mild proteinuria. Monoclonal antibodies directed at human T lymphocyte subsets termed OKT3, OKT4 and OKT8 were used in an indirect immunofluorescence assay in all cases. Patients with minimal change nephropathy, with or without nephrotic syndrome and patients with IgA glomerulonephritis showed mean values of OKT3+ cells (total peripheral T cells), helper OKT4+ cells, suppressor OKT8+ cells and OKT4+/OKT8+ cell ratio, in the normal range. Only the group of patients with membranous glomerulonephritis and nephrotic syndrome presented a mean OKT4+/OKT8+ ratio greater than the normal group (percentages: 2·43±0·3 vs 1·6±0·1 s.e.m.; P<0·02). This increased ratio was due to a reduction in the OKT8+ cell subset compared to the healthy subjects (percentages: 27·6±2·9 vs 36·8±1·4 s.e.m.; P<0·01). Our data shows that the functional lymphocyte disorders previously described in minimal change nephropathy and IgA glomerulonephritis are not due to a numerical imbalance of lymphocyte subsets. Such an imbalance of lymphocyte subsets was specifically observed in membranous glomerulonephritis with nephrotic syndrome. The true significance of this finding has to be clarified by longitudinal studies and functional tests.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chatenoud L., Bach M. A. Abnormalities of T-cell subsets in glomerulonephritis and systemic lupus erythematosus. Kidney Int. 1981 Aug;20(2):267–274. doi: 10.1038/ki.1981.130. [DOI] [PubMed] [Google Scholar]
- Dutton R. W. Suppressor T cells. Transplant Rev. 1975;26:39–55. doi: 10.1111/j.1600-065x.1975.tb00174.x. [DOI] [PubMed] [Google Scholar]
- Evans D. J. Pathogenesis of membranous glomerulonephritis. Lancet. 1974 Jun 8;1(7867):1143–1144. doi: 10.1016/s0140-6736(74)90624-2. [DOI] [PubMed] [Google Scholar]
- Moretta L., Mingari M. C., Moretta A. Hunan T cell subpopulations in normal and pathologic conditions. Immunol Rev. 1979;45:163–193. doi: 10.1111/j.1600-065x.1979.tb00277.x. [DOI] [PubMed] [Google Scholar]
- Ooi B. S., Ooi Y. M., Hsu A., Hurtubise P. E. Diminished synthesis of immunoglobulin by peripheral lymphocytes of patients with idiopathic membranous glomerulonephropathy. J Clin Invest. 1980 Apr;65(4):789–797. doi: 10.1172/JCI109729. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reinherz E. L., Moretta L., Roper M., Breard J. M., Mingari M. C., Cooper M. D., Schlossman S. F. Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. J Exp Med. 1980 Apr 1;151(4):969–974. doi: 10.1084/jem.151.4.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakai H., Nomoto Y., Arimori S. Decrease of IgA-specific suppressor T cell activity in patients with IgA nephropathy. Clin Exp Immunol. 1979 Nov;38(2):243–248. [PMC free article] [PubMed] [Google Scholar]
- Sasdelli M., Cagnoli L., Candi P., Mandreoli M., Beltrandi E., Zucchelli P. Cell mediated immunity in idiopathic glomerulonephritis. Clin Exp Immunol. 1981 Oct;46(1):27–34. [PMC free article] [PubMed] [Google Scholar]
- Shalhoub R. J. Pathogenesis of lipoid nephrosis: a disorder of T-cell function. Lancet. 1974 Sep 7;2(7880):556–560. doi: 10.1016/s0140-6736(74)91880-7. [DOI] [PubMed] [Google Scholar]
- Van Damme B. J., Fleuren G. J., Bakker W. W., Vernier R. L., Hoedemaeker P. J. Experimental glomerulonephritis in the rat induced by antibodies directed against tubular antigens. V. Fixed glomerular antigens in the pathogenesis of heterologous immune complex glomerulonephritis. Lab Invest. 1978 Apr;38(4):502–510. [PubMed] [Google Scholar]
- Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]