Table 4.
Frequency of specific types of base substitution mutations generated during in vitro gap-filling replication by pol V and by pol III holoenzyme
| Mutation | Mismatch* | Mutation frequency × 10−5 per gene
|
Pol V/Pol III | |
|---|---|---|---|---|
| Pol III | Pol V | |||
| Transition | ||||
| A → G | A⋅C | <1.4 | <23.0 | — |
| C → T | C⋅A | 15.2 | 115.1 | 8 |
| G → A | G⋅T | 2.8 | 46.0 | 16 |
| T → C | T⋅G | <1.4 | 368.3 | >263 |
| Transversion | ||||
| A → C | A⋅G | 2.8 | 161.1 | 58 |
| A → T | A⋅A | <1.4 | 414.4 | >296 |
| C → A | C⋅T | <1.4 | 92.1 | >66 |
| C → G | C⋅C | <1.4 | 23.0 | >16 |
| G → C | G⋅G | 6.9 | 115.1 | 17 |
| G → T | G⋅A | 2.8 | 23.0 | 8 |
| T → A | T⋅T | 2.8 | 230.2 | 82 |
| T → G | T⋅C | <1.4 | 69.1 | >49 |
Mutation frequency was calculated based on the data in Tables 1 and 2. The mismatches formed most frequently by pol V, and their frequencies are underlined. The largest differences between pol V and pol III holoenzyme are in boldface type.
*
The mismatches that gave rise to the observed mutations. The template nucleotide in each pair is shown first.