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. 1981 Mar;43(3):583–589.

The in vitro response to mitogens of leucocytes from cattle infected with Trypanosoma congolense.

R A Masake, T W Pearson, P Wells, G E Roelants
PMCID: PMC1537187  PMID: 7285394

Abstract

The in vitro proliferative response of bovine leucocytes from peripheral blood to LPS, PWM and Con A was monitored during the course of infection with T. congolense. The stimulatory effect of LPS and PWM on PBL was significantly decreased in infected cattle while the reduction of stimulatory response to Con A was less marked. There was a high background proliferation of unstimulated leucocytes from infected cattle. This increase in background counts correlated with the parasitaemia in that both features were abolished by treatment with the trypanocidal drug Berenil (4,4'-diamidino-diazoaminobenzene-diacetamide acetone). Co-cultivation of trypanosomes with PBL from normal cattle did not depress the responses of the latter to mitogens, and trypanosomes maintained in a cell-free medium failed to incorporate 14C-thymidine under the conditions used.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albright J. F., Albright J. W., Dusanic D. G. Trypanosome-induced splenomegaly and suppression of mouse spleen cell responses to antigen and mitogens. J Reticuloendothel Soc. 1977 Jan;21(1):21–31. [PubMed] [Google Scholar]
  2. Andersson J., Grönvik K. O., Larsson E. L., Coutinho A. Studies on T lymphocyte activation. I. Requirements for the mitogen-dependent production of T cell growth factors. Eur J Immunol. 1979 Aug;9(8):581–587. doi: 10.1002/eji.1830090802. [DOI] [PubMed] [Google Scholar]
  3. Assoku R. K., Tizard I. R. Mitogenicity of autolysates of Trypanosoma congolense. Experientia. 1978 Jan 15;34(1):127–129. doi: 10.1007/BF01921943. [DOI] [PubMed] [Google Scholar]
  4. Corsini A. C., Clayton C., Askonas B. A., Ogilvie B. M. Suppressor cells and loss of B-cell potential in mice infected with Trypanosoma brucei. Clin Exp Immunol. 1977 Jul;29(1):122–131. [PMC free article] [PubMed] [Google Scholar]
  5. Goodwin L. G., Green D. G., Guy M. W., Voller A. Immunosuppression during trypanosomiasis. Br J Exp Pathol. 1972 Feb;53(1):40–43. [PMC free article] [PubMed] [Google Scholar]
  6. Jayawardena A. N., Waksman B. H., Eardley D. D. Activation of distinct helper and suppressor T cells in experimental trypanosomiasis. J Immunol. 1978 Aug;121(2):622–628. [PubMed] [Google Scholar]
  7. Jayawardena A. N., Waksman B. H. Suppressor cells in experimentally trypanosomiasis. Nature. 1977 Feb 10;265(5594):539–541. doi: 10.1038/265539a0. [DOI] [PubMed] [Google Scholar]
  8. Lanham S. M. Separation of trypanosomes from the blood of infected rats and mice by anion-exchangers. Nature. 1968 Jun 29;218(5148):1273–1274. doi: 10.1038/2181273a0. [DOI] [PubMed] [Google Scholar]
  9. Luckins A. G., Mehlitz D. Observations on serum immunoglobulin levels in cattle infected with Trypanosoma brucei, T. vivax and T. congolense. Ann Trop Med Parasitol. 1976 Dec;70(4):479–480. doi: 10.1080/00034983.1976.11687153. [DOI] [PubMed] [Google Scholar]
  10. Murray M., Morrison W. I. Non-specific induction of increased resistance in mice to Trypanosoma congolense and Trypanosoma brucei by immunostimulants. Parasitology. 1979 Dec;79(3):349–366. doi: 10.1017/s0031182000053750. [DOI] [PubMed] [Google Scholar]
  11. Murray M., Murray P. K., McIntyre W. I. An improved parasitological technique for the diagnosis of African trypanosomiasis. Trans R Soc Trop Med Hyg. 1977;71(4):325–326. doi: 10.1016/0035-9203(77)90110-9. [DOI] [PubMed] [Google Scholar]
  12. Murray P. K., Jennings F. W., Murray M., Urquhart G. M. The nature of immunosuppression in Trypanosoma brucei infections in mice. II. The role of the T and B lymphocytes. Immunology. 1974 Nov;27(5):825–840. [PMC free article] [PubMed] [Google Scholar]
  14. Pearson T. W., Roelants G. E., Lundin L. B., Mayor-Withey K. S. Immune depression in trypanosome-infected mice. I. Depressed T lymphocyte responses. Eur J Immunol. 1978 Oct;8(10):723–727. doi: 10.1002/eji.1830081010. [DOI] [PubMed] [Google Scholar]
  15. Pearson T. W., Roelants G. E., Lundin L. B., Mayor-Withey K. S. The bovine lymphoid system: binding and stimulation of peripheral blood lymphocytes by lectins. J Immunol Methods. 1979;26(3):271–282. doi: 10.1016/0022-1759(79)90252-7. [DOI] [PubMed] [Google Scholar]
  16. Roelants G. E., Pearson T. W., Morrison W. I., Mayor-Withey K. S., Lundin L. B. Immune depression in trypanosome-infected mice. IV. Kinetics of suppression and alleviation by the trypanocidal drug Berenil. Clin Exp Immunol. 1979 Sep;37(3):457–469. [PMC free article] [PubMed] [Google Scholar]
  17. Sollod A. E., Frank G. H. Bovine trypanosomiasis: effect on the immune response of the infected host. Am J Vet Res. 1979 May;40(5):658–664. [PubMed] [Google Scholar]

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