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. 2003 May;15(5):1159–1169. doi: 10.1105/tpc.009506

Figure 3.

Figure 3.

Effects of MAF2 Overexpression in the Columbia Accession.

(A) 35S:MAF2 plants are late flowering and do not respond to vernalization. Plants are shown after ∼50 days of growth under a 12-h photoperiod after a 76-day 4°C cold treatment of the imbibed seeds.

(B) Expression of FLC, MAF2, and SOC1 in wild-type Columbia, 35S:MAF2, and 35S:FLC seedlings. RNA was extracted from pools of 10 whole seedlings after 10 days of growth under 12 h of light after either a 3-day cold stratification (−; nonvernalized) or a 76-day vernalization treatment (+; vernalized). Expression was monitored by RT-PCR over 20, 25, 30, and 35 cycles. Products are shown after 25 cycles. No significant changes in FLC mRNA levels are apparent in the 35S:MAF2 samples relative to the wild-type control, whereas SOC1 levels are reduced in the 35S:MAF2 plants in a manner comparable to that observed in the 35S:FLC samples.