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. 2000 Jan 18;97(2):583–587. doi: 10.1073/pnas.97.2.583

Figure 3.

Figure 3

Combined reverse-phase HPLC-atmospheric pressure chemical ionization MS analysis of the biosynthetic product (retention time = 8.6 ± 0.1 min) generated by the recombinant acetyl transferase with 10-DAB and acetyl CoA as cosubstrates (Upper spectrum) and of authentic baccatin III (retention time = 8.6 ± 0.1 min; Lower spectrum). The diagnostic mass spectral fragment ions are at m/z 605 (P + NH4+), 587 (PH+), 572 (PH+ − CH3), 527 (PH+ − CH3COOH), and 509 (PH+ − (CH3COOH − H2O). For column, solvent, flow rates, and instrument details, see Materials and Methods.