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. 2003 Apr 15;31(8):2209–2216. doi: 10.1093/nar/gkg303

Figure 5.

Figure 5

Interaction of Hap46 with transcription factors. Fusion proteins of GST with full-length Hap46 (lanes 2) or Hap46ΔC47 (lanes 3) were attached to GSH–Sepharose and used as affinity matrices for in vitro expressed radiolabeled transcription factors, as described in Materials and Methods. For controls, plain GSH–Sepharose was used (lanes 1). Rabbit reticulocyte lysate was used as such in the experiment of (A). Eluates were analyzed by SDS–PAGE followed either by autoradiographic detection of radiolabeled bands or, in the case of hsc70 (A), by specific immunoblotting.