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Interaction of Hap46 and hsc70 with the estrogen receptor. The estrogen receptor attached to a specific immunomatrix was incubated with the GST–Hap46 fusion protein, as described in Materials and Methods. The experiment of lane 2 contained minimal amounts of endogenous hsc70 co-purified with the receptor, while further hsc70 (5 µg) was added in the experiment of lane 3. As control (lane 1), the plain immunomatrix was used without estrogen receptor but with hsc70 (5 µg) added.
