Figure 4.

Typical autoradiograms of a 10% PAGE of the L3 loop probing. (A) The ribozyme with the original sequence. (B) The G₂₇C₁₂/C₂₈G₁₁ P1.1 mutant. Both ribozymes were pre-incubated in the presence of the SdC4 analog prior to the addition of MgCl₂. Lanes 1 are aliquots of ribozyme and SdC4 analog removed at 0 min and treated with RNase H in the absence of any oligonucleotide. Lanes 2, 3 and 4 are aliquots removed at 0, 1 and 5 min, respectively, and treated with the RNase H in the presence of an oligonucleotide. The position of the xylene cyanol (XC) is indicated.