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Fluorescence decays recorded from H-258 solutions in 50 mM phosphate buffer (pH 7.0) bound to DNA at different [H-258]/[DNA bp] ratios of 0.05 (10 µM) (a), 0.15 (30 µM) (b) and 0.20 (40 µM) (c) and different detection wavelengths of 440, 460, 480 and 500 nm. With increasing detection wavelength the fluorescence lifetime increases. In the case of a [H-258]/[DNA bp] ratio of 0.05 the fluorescence decay recorded at 500 nm can be fitted almost mono-exponentially.
