Figure 5.

Protein-binding assay. (A) Competitive mobility shift assay showing the ability of unlabeled oligonucleotides to compete with radiolabeled TEL for binding to nuclear proteins. The band marked by an asterisk indicates the specific GRO-binding protein (thought to be nucleolin). The names of the oligonucleotides are abbreviated without the KS or GRO prefixes. (B) Plot of the relative intensity of the protein band in (A) versus the relative number of viable cells remaining after treatment with oligonucleotide annealed in KCl buffer. The squared correlation coefficient (R²) for the relationship between these variables is 0.82. The names of the oligonucleotides are abbreviated without the KS or GRO prefixes.