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Constitutive overexpression of Ebp1 in LNCaP cells affects induction of cell cycle regulated genes in response to serum. LNCaP cells stably transfected with either pcDNA3 or ebp1 were serum starved for 24 h and then serum stimulated for up to 28 h as indicated before RNA harvesting. Northern blots were prepared using 30 µg of total RNA from each cell line. The blots were probed with 32P-labeled cDNA for the indicated genes and were visualized by autoradiography.
