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. 2003 Apr 15;31(8):2025–2034. doi: 10.1093/nar/gkg324

Figure 4.

Figure 4

Examination of self-association of Mlh1(343) and Pms1(396) in solution. Glutaraldehyde cross-linking reactions were performed as described in Materials and Methods and visualized by SDS–PAGE. The absence or presence of glutaraldehyde (0.0025%) is indicated above each lane. Protein molar concentrations in the reactions were 535 nM Mlh1–Pms1 heterodimer, 840 nM Mlh1, 3.9 µM Mlh1(343) and 3.8 µM Pms1(396). Molecular weight standards were from Bio-Rad (broad range). Under some conditions involving protein plus DNA combinations mentioned in Results, we did observe a faint ladder of bands whose intensity and mobility suggested non-specific cross-linking of protein molecules bound to adjacent DNA binding sites (data not shown).