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Low-temperature resonance Raman spectra of transient [2Fe-2S]2+ clusters. (a) NifU(Asp37Ala) after treatment by the cluster biosynthetic system; (b) as-isolated NifU(Asp37Ala); (c) difference spectrum (spectrum a minus spectrum b); (d) NifU-1(Asp37Ala) after treatment with the cluster biosynthetic system. The conditions used are the same as those described in Fig. 4, and each spectrum is the sum of 33 scans.
