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. 2003 Mar 15;111(6):877–885. doi: 10.1172/JCI15645

Figure 3.

Figure 3

Sublytic C5b-9 does not induce apoptosis in podocytes. a–f show TUNEL staining, a marker of apoptosis. Apoptosis was not detected in control rat (a) and mouse (d) podocytes exposed to Ab without a complement source, nor in experimental rat (b) and mouse (e) cells exposed to Ab with a complement source. In contrast, TUNEL staining is increased in rat (c) and mouse (f) podocytes exposed to UV irradiation used as a positive control. The suicide track DNA ladder assay is shown in rat (g) and mouse (h) podocytes. In contrast to DNA fragmentation in podocytes exposed to UV irradiation (lanes 1, 2, 3), DNA fragmentation was not detected in cells exposed to Ab with (C+) (lanes 4, 5, 6) and without (C–) (lanes 7, 8, 9) a complement source.