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. 2003 Mar 15;111(6):877–885. doi: 10.1172/JCI15645

Figure 6.

Figure 6

ERK regulates p21 and GADD45 following sublytic C5b-9 attack. (a) Phosphorylated ERK was measured by Western blot analysis in control (C–) and C5b-9–attacked (C+) podocytes, and (b) densitometry was used to quantitate the changes compared with total ERK levels. Phosphorylated ERK increased within 15 min of sublytic C5b-9 attack, and this normalized by 1 h. This was followed by a second increase in phosphorylated ERK at 8 h. (c) Inhibiting phospho-ERK with U0126 reduced GADD45 and p21. (d) Inhibiting ERK-1 and -2 with U0126 augments the DNA damage induced by sublytic C5b-9 (lane 4) compared with controls (lane 3).