Table 1.
S8-binding site variations at 597-643/641 and their corresponding growth rates and Kds
| (597-643)/641 | No. isolates | Doubling time, min | Kd | |
|---|---|---|---|---|
| Class I | G-C/U | 3 | 70 | 1 |
| C-G/A | 7 | 70 | 2 | |
| U-G/C | 3 | 70 | 1 | |
| G-C/A | 3 | 70 | 1 | |
| C-A/A | 8 | 70 | 7.5 | |
| Class II | C-G/U | 4 | 160 | 10 |
| C-G/C | 5 | 140 | 10 | |
| C-C/A | 3 | 165 | 50 | |
| U-A/G | 10 | 135 | 40 | |
| U-G/G | 4 | 150 | 100 | |
| U-A/A | 10 | 160 | 100 | |
| C-U/A | 1 | 165 | >100 | |
| Class III | A-C/C | 1 | 300 | 10 |
| C-G/G | 17 | 400 | 100 | |
| U-G/A | 2 | >400 | >100 | |
| G-G/A | 2 | >400 | >100 | |
| A-A/G | 2 | >400 | >100 | |
| G-A/G | 1 | 400 | ND | |
| U-A/C | 1 | >400 | ND | |
| C-A/U | 1 | >400 | ND | |
| U-G/U | 1 | >400 | ND | |
| U-A/C | 1 | >400 | ND | |
| A-A/A | 2 | >400 | ND |
The number of isolates for each variant is indicated (for a total of 92 clones sequenced). Growth rate values reflect the mean of at least three independent dt measurements done with different isolates. Standard deviation is ±25%. Kd values are relative to the wt value of 3 nM for rRNA transcript 576-765 with standard deviation being ±10%. ND, not determined.