FIG. 5.
Ebola virus GP does not induce cell death. Floating cells (3 × 105 per well) from 293T cells 48 h posttransfection were replated into fresh six-well plates. At 24-h intervals floating cells were counted (•) and then pooled with versine-detached adherent cells, and viable (♦) and nonviable (▪) cell numbers were estimated using trypan blue exclusion. Cells were then adhered to poly-d-lysine-coated plates, and the percentage of Ebola virus GP-positive cells (▴) was quantified using indirect immunofluorescence staining with KZ52. Values represent the geometric means of triplicate samples ± standard errors. Data are representative of three independent experiments.