FIG. 4.

(A) Genomic structure of Apof. The dashed line indicates the intron sequence. Boxes represent the sequence of the mRNA: the open box represents the transcribed but untranslated regions; the gray box represents amino acids that are absent from the mature ApoF protein; the black box represents the mature ApoF protein. The position of the signal peptide is indicated by an arrow. The three glycosylation sites and four hydrophobic domains are indicated by stars and dotted lines, respectively. (B) PCR primers and predicted products. (C) Gel electrophoresis of PCR products. N, DNA from LVIF-negative (NFS) mice; C, DNA from LVIF-positive (CAST) mice.