TABLE 1.
Mouse strain | Mean % antigen-positive cells ± SD at:
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1 wk postinfectionb in:
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2 wks postinfectionc in:
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Spleen | Bone marrow | Blood cells | Spleen | Bone marrow | Blood cells | ||
B6 | 7.9 ± 2.6 | 11.6 ± 6.6 | 0.5 ± 0.3d | 12.8 ± 8.9 | 20.4 ± 6.8 | 0.5 ± 0.3d | |
B6.IFN-γ−/− | 11.4 ± 5.3 | 22.1 ± 9.4 | 6.0 ± 3.8 | 1.5 ± 2.1 | 4.3 ± 8.4 | 1.5 ± 2.0 |
All tissues were examined by flow cytometry as single-cell suspensions of nucleated, live cells. The numbers of mice in each group were as follows at 1 week postinfection: B6.IFN-γ−/− (n = 6) and B6 (n = 6). The numbers of mice in each group were as follows at 2 weeks postinfection: B6.IFN-γ−/− (n = 11) and B6 (n = 15).
P = 0.3095, 0.0256, and 0.0002 for spleen, bone marrow, and blood cells, respectively.
P = <0.0001, 0.0006, and 0.6070 for spleen, bone marrow, and blood cells, respectively.
The percentage of cells that stained positive for antigen in the infected B6 mice was below or equivalent to the background staining in naive mice.