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Schematic diagram of plasmids used to construct recombinant baculoviruses expressing LEF-1 and LEF-2. The two oligomers used to engineer the plasmids pHSEpiHisLef-1 and pHSEpiHisLef-2 (45) are shown. They were inserted into a BglII site and resulted in the elimination of this site and the creation of an upstream BamHI site. The inserts were then removed with BamHI and NotI and inserted into pFastBac1 as shown. The orientation of a generic lef gene is indicated by the open arrow.
