FIG. 6.

RNA synthesis catalyzed by *LEF-1 on single-stranded DNA of phage M13mp9. Reactions were conducted as described in Materials and Methods. Aliquots were removed after 30, 60, and 120 min. Prior to electrophoresis in a 1.2% agarose gel, each sample was divided in two portions. The first portion was loaded directly onto the gel (lanes 1 to 4), while the second portion was loaded after heating for 3 min at 100°C (lanes 5 to 8). After electrophoresis, the gel was transferred onto Whatman 3MM paper, dried under vacuum, and exposed to X-ray film. M13 DNA and an RNA ladder were loaded onto separate lanes and visualized by staining with ethidium bromide. The migration of M13 DNA and RNA markers is indicated on the left and right sides of the gel, respectively.