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. 2002 Mar;76(5):2329–2339. doi: 10.1128/jvi.76.5.2329-2339.2002

FIG. 2.

FIG. 2.

Replication of wild-type (wt), mutant, and revertant HIV-1 viruses. (A) SupT1 cells were transfected with 1 μg of the proviral constructs. CA-p24 production was measured in the culture medium at several days posttransfection. (B) Increased replication of the 2L virus after 3.5 months of culturing. Virus samples obtained from cultures 1 and 2 (corresponding to rev1 and rev2, respectively) were assayed for their replication capacities by infection of SupT1 cells with the same amount of virus (10 ng of CA-p24). (C) Increased replication capacity of the mutant d1 virus upon prolonged culturing. Virus samples obtained from d1 cultures A and B after 4 and 10 weeks were assayed for their replication capacities by infection of fresh SupT1 cells with the same amount of virus (10 ng of CA-p24). (D) Increased replication capacity of the mutant d1/2 virus in cultures J and K after 4 and 10 weeks of culturing.