FIG. 6.

Relative reverse transcription activities of the wild-type and mutant templates. (A) Reverse transcription activity of virion-isolated vRNA-tRNA complexes as measured in Fig. 5 (ex vivo method). The results of three independent experiments were quantitated. There is a significant standard deviation for the d2 sample, which is due to the severe virus production defect of this mutant. The activity of the wild-type (wt) template was arbitrarily set at 1. For comparison, the results obtained previously with in vitro-assembled vRNA-tRNA complexes are included (8). (B) The tRNA occupancy of the PBS is shown for the wild-type and mutant vRNA genomes. The 374- and 175-nt products for three independent experiments were quantitated. The total of these products was arbitrarily set at 100%, and the percentage of 175-nt product is plotted.