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. 2002 Mar;76(5):2329–2339. doi: 10.1128/jvi.76.5.2329-2339.2002

FIG. 8.

FIG. 8.

Reverse transcription with the wild-type and RNase H-negative RT enzyme. vRNA-tRNA complexes isolated from wild-type, d1, and d1 rev1 virions were used as templates for extension of the downstream AUG DNA primer (lanes 7 to 12). Reverse transcription was performed with the wild-type HIV-1 RT enzyme (lanes 7 to 9) and the RNase H-negative RT enzyme (RH− RT; lanes 10 to 12). The major stop product (stop) on the d1 template that is observed upon extension of the AUG primer with wild-type RT (lane 8) is not observed with RNase H-negative RT (lane 11). AUG-primed reverse transcription with the RNase H-negative RT did not yield the full-length 374-nt product but produced several stop products. This is probably caused by the reduced processivity of the mutant RT enzyme. Control reactions were performed with the poly(A) primer (lanes 1 to 3) and the tRNA primer (lanes 4 to 6).