FIG. 4.
Presence of MAdCAM-1+ reticular endothelial cells in the splenic marginal zone and of ERTR-9+ marginal zone macrophages but lack (or much less) of N418-positive dendritic cells is associated with extensive early virus production in the spleen following LCMV-WE infection (500 PFU, i.v.). Representative examples of spleen sections from C57BL/6 mice at 24 h postinfection (5 × 103 PFU of LCMV per g of spleen) (A and B) and 4 days postinfection (8.2 × 106 PFU of LCMV per g of spleen) (C and D), from a RAG2−/− mouse (7 × 104 PFU of LCMV per g of spleen) (E) and an LTα−/− mouse (8 × 104 PFU of LCMV per g of spleen) (F) at 4 days postinfection, and from a RAG2−/− mouse (1.6 × 103 PFU of LCMV per g of spleen) (G) and a C57BL/6 mouse (1.5 × 105 PFU of LCMV per g of spleen) (H) at 48 h postinfection, with immunostaining for MAdCAM-1 (A, C, E, and F), ERTR-9 (B and D), or CD11c (N418) (G and H) (positive reaction in red), were hybridized in situ with 35S-labeled RNA probes specific for LCMV GP (mRNA-positive cells, black silver grains). Magnifications, ×125 (A to D) and ×250 (E to H).