TABLE 1.
Morphometric analysis of spleen sections hybridized with an LCMV GP-specific mRNA probe and amounts of virus isolated from the corresponding spleensa
Mouse strainb | % Black area of total tissue | LCMV CPFU/g of spleen |
---|---|---|
C57BL/6, uninfected | 0 | 0 |
C57BL/6 | 22.2 | 5.4 × 106 |
RAG2−/− | 0.2 | 4.7 × 104 |
TNF-α−/− | 9.0 | 9.8 × 105 |
LTα−/− | 0.9 | 7.4 × 104 |
TNF-α−/− LTα−/− | 0.2 | 3.7 × 104 |
Longitudinally cut spleen tissue samples from individual mice were frozen for subsequent in situ hybridization or used for LCMV plaque assays. The fraction of tissue area covered by black silver grains upon hybridization with an LCMV GP-specific RNA probe was determined by computer-assisted morphometric analysis.
Samples were taken 4 days after LCMV infection unless otherwise noted.