Skip to main content
. 2002 Mar;76(5):2410–2423. doi: 10.1128/jvi.76.5.2410-2423.2002

FIG. 7.

FIG. 7.

Transcriptional direction of the RNAs between the 5"HS5 LTR and HS5 site. (a) The RNAs transcribed from DNA between the 5"HS5 LTR and HS5 site of the β-LCR were exclusively in the sense direction in nontransfected K562 cells as determined by RT-PCRs. Horizontal left-to-right arrow, sense RNAs transcribed from the LTR into the HS5 site as amplified with primer pairs 1 to 5. Numbers below the PCR products, sizes of the RT-PCR bands in base pairs. (b) Gel electrophoresis of RT-PCR products synthesized with locus-specific primers. Lanes 1 to 5, PCR bands amplified from cDNAs by primer pairs 1 to 5, respectively. + lanes, PCR bands generated from the sense R RNAs using the reverse primer of primer pairs 1 to 5 in the RT step. − lanes, PCR bands generated from the antisense RNAs using the forward primers of primer pairs 1 to 5 in the RT step. White dots on the right margins, PCR bands anticipated to be generated by primer pairs 1 to 5. (c) PCR products amplified from cDNAs synthesized with random hexamer primers in the RT step. Lanes 1 to 5, PCR bands amplified from the cDNAs by primer pairs 1 to 5, respectively.