FIG. 3.

BALF1 does not protect a lymphocyte cell line against BAX-induced death. (A) DG75 cells were transiently cotransfected with plasmids encoding the indicated proteins with N-terminal HA tags as well as 0.5 μg of EGFP to mark transfected cells. Apoptosis was assessed at 18 h posttransfection by measuring the loss of mitochondrial membrane potential (CMX-Ros fluorescence) in GFP-expressing cells. (B) Graphic presentation of the mean ± SEM for three independent experiments as shown in panel A. Shown below is the amount (in micrograms) of plasmid DNA transfected (with all samples receiving equal amounts of DNA balanced with empty vector) and a corresponding immunoblot analysis.