FIG. 6.
BALF1 binds to itself and BHRF1 in vitro. (A) COS-1 cells were transfected with plasmids expressing the indicated HA-tagged BCL-2 family members plus either FLAG-BALF1 or FLAG-BCL-xL as a positive control. Anti-HA immunoprecipitates and total cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotted with the antibodies indicated on the right of each panel. A representative immunoprecipitation is shown (n = 3). (B, C, and D) Coimmunoprecipitation experiments were performed with lysates of COS-1 cells transfected with the indicated plasmids, as described for panel A.