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. 1975 Feb;19(2):309–318.

Cell damage and dye reduction in the quantitative nitroblue tetrazolium (NBT) test.

A W Segal, A J Levi
PMCID: PMC1538095  PMID: 1212802

Abstract

Nitroblue tetrazolium (NBT) is toxic to neutrophils; an effect which is greatly enhanced by endotoxin and latex particles. Cell damage, measured by the release of the cytoplasmic marker enzyme lactate dehydrogenase (LDH), was closely related to dye reduction. This suggests that, in this test, dye reduction occurs largely as a result of contact between intracellular reducing compounds and NBT following damage of the outer cell membrane. The expression of dye reduction as a function of LDH release should enhance the sensitivity of the quantitative NBT test by correcting for the observed intersubject variation in cell damage. The relationship between cell damage and dye reduction is a measure of the reducing capacity of the cell. This was normal in immature, bone marrow neutrophils, but diminished in neutrophils of patients with chronic granulomatous disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashburn P., Cooper M. R., McCall C. E., DeChatelet L. R. Nitroblue tetrazolium reduction. False positive and false negative results. Blood. 1973 Jun;41(6):921–925. [PubMed] [Google Scholar]
  2. Baehner R. L., Nathan D. G. Leukocyte oxidase: defective activity in chronic granulomatous disease. Science. 1967 Feb 17;155(3764):835–836. doi: 10.1126/science.155.3764.835. [DOI] [PubMed] [Google Scholar]
  3. Baehner R. L., Nathan D. G. Quantitative nitroblue tetrazolium test in chronic granulomatous disease. N Engl J Med. 1968 May 2;278(18):971–976. doi: 10.1056/NEJM196805022781801. [DOI] [PubMed] [Google Scholar]
  4. Blackburn M. J., Andrews T. M., Watts R. W. Measurement of DNA in isolated granulocytes by the ethidium technique. Anal Biochem. 1973 Jan;51(1):1–10. doi: 10.1016/0003-2697(73)90446-6. [DOI] [PubMed] [Google Scholar]
  5. Cocchi P., Mori S., Becattini A. N.B.T. tests in premature infants. Lancet. 1969 Dec 27;2(7635):1426–1427. doi: 10.1016/s0140-6736(69)90970-2. [DOI] [PubMed] [Google Scholar]
  6. Davies P., Fox R. I., Polyzonis M., Allison A. C., Haswell A. D. The inhibition of phagocytosis and facilitation of exocytosis in rabbit polymorphonuclear leukocytes by cytochalasin B. Lab Invest. 1973 Jan;28(1):16–22. [PubMed] [Google Scholar]
  7. De la Vega G., Freyre-Horta R., Benitez-Bibriesca L. Plasma factor affecting the NBT reducing capacity of neutrophils. N Engl J Med. 1973 Aug 2;289(5):271–272. doi: 10.1056/nejm197308022890516. [DOI] [PubMed] [Google Scholar]
  8. Gifford R. H., Malawista S. E. A simple rapid micromethod for detecting chronic granulomatous disease of childhood. J Lab Clin Med. 1970 Mar;75(3):511–519. [PubMed] [Google Scholar]
  9. Klebanoff S. J. Intraleukocytic microbicidal defects. Annu Rev Med. 1971;22:39–62. doi: 10.1146/annurev.me.22.020171.000351. [DOI] [PubMed] [Google Scholar]
  10. Nathan D. G. Editorial: NBT reduction by human phagocytes. N Engl J Med. 1974 Jan 31;290(5):280–281. doi: 10.1056/NEJM197401312900512. [DOI] [PubMed] [Google Scholar]
  11. Park B. H., Fikrig S. M., Smithwick E. M. Infection and nitroblue-tetrazolium reduction by neutrophils. A diagnostic acid. Lancet. 1968 Sep 7;2(7567):532–534. doi: 10.1016/s0140-6736(68)92406-9. [DOI] [PubMed] [Google Scholar]
  12. Segal A. W., Levi A. J. The mechanism of the entry of dye into neutrophils in the nitroblue tetrazolium (NBT) test. Clin Sci Mol Med. 1973 Dec;45(6):817–826. doi: 10.1042/cs0450817. [DOI] [PubMed] [Google Scholar]
  13. Thompson E. N., Soothill J. F. Chronic granulomatous disease: quantitative clinicopathological relationships. Arch Dis Child. 1970 Feb;45(239):24–32. doi: 10.1136/adc.45.239.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Windhorst D. B., Holmes B., Good R. A. A newly defined X-linked trait in man with demonstration of the Lyon effect in carrier females. Lancet. 1967 Apr 8;1(7493):737–739. doi: 10.1016/s0140-6736(67)91360-8. [DOI] [PubMed] [Google Scholar]
  15. Xanthou M. Leucocyte blood picture in ill newborn babies. Arch Dis Child. 1972 Oct;47(255):741–746. doi: 10.1136/adc.47.255.741. [DOI] [PMC free article] [PubMed] [Google Scholar]

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