CTL responsiveness after stimulation of PBMC cryopreserved between 1982 and 1984 with influenza A viruses (H3N2) containing the NP418-426/1980 or NP418-426/1972 variant epitope. PBMC from two HLA-A2+ HLA-B35+ donors (donors 1 and 2) were stimulated in vitro with A/Victoria/3/75 (A and C), containing the NP418-426/1972 variant epitope, or Resvir-9 (B and D), containing the NP418-426/1980 variant epitope. The lytic activities of the effector cells were determined after 8 days of culture against target cells incubated with 5 μM concentrations of peptides corresponding to NP418-426/1980 (▿), NP418-426/1972 (□), NP418-426/1957 (⧫), or M158-66 epitope (▵), and a negative control (•) at different effector-to-target cell ratios. NP418-426/1957 was not determined for PBMC of donor 2 after stimulation with Resvir-9. The mean percentage of specific lysis ± standard deviation is given for one experiment.