FIG. 2.

Evaluation of hHyal2, oHyal2, or bHyal2 proteins as receptors for ENTV and JSRV. HT-1080 and NIH 3T3 cells were seeded at a density of 5 × 10⁵/dish in 60-mm-diameter dishes. After 24 h, the cells were transfected with expression plasmids encoding hHyal2, oHyal2, or bHyal2 or an empty expression plasmid (none). The following day, the transfected cells were trypsinized (1.5 ml of trypsin), and 100-μl aliquots of each preparation were seeded in the wells of six-well dishes. SSF cells were also seeded at a density of 10⁵/well in six-well dishes. After 24 h, the cells were exposed to the ENTV or JSRV vectors. Two days after vector exposure, the cells were stained for AP⁺ foci. Results are means of data from two to four experiments, each done in duplicate. nd, not done.