FIG. 4.

Both phosphorylated and nonphoshorylated forms of STAT1 are degraded by SV5. 2fTGH cells were or were not pretreated with the kinase inhibitor genistein (100 μM) for 1 h prior to being mock infected or infected with SV5 at an MOI of 10. Cells were or were not cultured in the presence of genistein for a further 6 h. Total cell extracts were made, and the presence of STAT1 was detected by immunoblot analysis with polyclonal anti-STAT1 antibody (a) or anti- phosphotyrosine (Y701) STAT1 [anti-STAT1 p-tyrosine (701)] (b).