FIG. 7.

Map of the TRL/IRL13 transcripts. (A) Northern blot analysis of RNA from HFF cells infected with wild-type or mutant viruses. The blot was hybridized with a ³²P-labeled TRL13-specific DNA. (B) RACE to amplify the 5" and 3" ends of TRL13- and IRL13-specific transcripts. At the top is a simplified diagram of the SMART RACE reaction (Clontech) with the GSPs and the universal primer mix (UPM) that anneals to the SMART oligonucleotides (Oligos) and modified oligo(dT) sequences. At the bottom are the bands amplified in the RACE PCRs; their size, relative to those of marker DNAs, are indicated.