Figure 6.

Location of targets of HxBPyne library on a phylogenic tree of the metalloprotease superfamily. With the exception of the carboxypeptidases, members from all major subfamilies of metalloproteases were targeted by the HxBPyne library. The tree was constructed from the peptidase domains of the complete human members of the metalloprotease clan, as designated by the MEROPS database⁴³ (205 total predicted members, 150 putative active members that possess conserved catalytic residues). A pairwise alignment was generated using ClustalW⁴⁴ and the phylogenic tree constructed using the neighbor-joining method⁴⁵. Major branches of the tree were designated by different colors, labeled by name, where appropriate, and displayed in hyperbolic space using the Hypertree program⁴⁶.