Table 1.
List of metalloprotease targets identified with HxBPyne probe library
| Metalloprotease | Mode of identification | Tissue or cell line | Average spectral counts |
|---|---|---|---|
| ADAM10 (IPI00013897) | LC-MS | MUM-2B | 9 |
| Gel | Recombinant | N/A | |
| ADAM17 (IPI00288894) | LC-MS | MDA-MB-231 | 4 |
| Gel | Recombinant | N/A | |
| ADAMTS4 (IPI00307276) | Gel | Recombinant | N/A |
| Adipocyte-derived leucine aminopeptidase | LC-MS | Mouse brain | 37 |
| (ALAP) (IPI00477831) | Gel | Mouse liver (cyto) | N/A |
| Gel | Recombinant COS7 | N/A | |
| AFG3-like protein 2 (IPI00001091) | LC-MS | MUM-2C | 13 |
| Alanyl aminopeptidase (AlaAP) (IPI00221224) | LC-MS | MUM-2B | 376 |
| Aspartyl aminopeptidase (IPI00072257) | LC-MS | MCF7 | 3 |
| Cystinyl aminopeptidase (IPI00221240) | LC-MS | MDA-MB-231 | 2 |
| Dipeptidylpeptidase 3 (DPPIII) (IPI00020672) | LC-MS | Mouse brain | 18 |
| Gel | Mouse liver (cyto) | N/A | |
| Endothelin-converting enzyme (IPI00002478) | LC-MS | MDA-MB-231 | 3 |
| Eupitrilysin (IPI00219613) | LC-MS | MUM-2B | 11 |
| Leucine aminopeptidase 3 (LAPIII) (IPI00331436) | Gel | Mouse liver (cyto) | N/A |
| Leukotriene A4 hydrolase (LA4H) (IPI00229527) | Gel | MDA-MB-231 | N/A |
| Gel | Recombinant COS7 | N/A | |
| MMP1 (IPI00110075) | LC-MS | Recombinant | 120a |
| MMP2 (IPI00112904) | LC-MS | Recombinant | 10a |
| MMP3 (IPI00123511) | Gel | Recombinant | N/A |
| MMP7(IPI00275232) | LC-MS | Recombinant | 3a |
| MMP9 (IPI00319200) | LC-MS | Recombinant | 8a |
| MMP12 (IPI00321648) | LC-MS | Recombinant | 8a |
| Neprilysin (IPI00247063) | LC-MS | MUM-2B | 27 |
| Paraplegin (IPI00299010) | LC-MS | MUM-2B | 2 |
| Puromycin-sensitive aminopeptidase | LC-MS | MDA-MB-231 | 31 |
| (PSAP) (IPI00026216) | Gel | Mouse liver (cyto) | N/A |
| Gel | Recombinant COS7 | N/A |
a
Spectral counts of 100 ng recombinant metalloprotease in 1 mg proteome.
N/A, not applicable; cyto, cytoplasm. Except where noted, spectral counts shown correspond to the proteome in which the highest level of each metalloprotease was found. For a complete list of ABPP-MudPIT results, see Supplementary Table 1.