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. 2006 Jul 27;25(15):3714–3724. doi: 10.1038/sj.emboj.7601246

Figure 1.

Figure 1

NFATc1 and calcineurin are ectopically expressed in pancreatic cancer. (A) Box-and-whisker plot illustrating normalized NFATc1 expression levels in pancreatic cancer (n=37) and normal/inflammatory control tissues (n=25) as determined in microarray experiments. (B) RT–PCR analyses using NFATc1-specific primers to validate the microarray data. Depicted are representative results for eight individual tissue samples. The human ribosomal protein, large, P0 gene (RPLP0) was used as housekeeping gene control. PaCa=pancreatic carcinoma; CP=chronic pancreatitis; NP=normal pancreas. (C) Serial sections of pancreatic cancer tissue stained for NFATc1 (upper panel) and calcineurin B (lower panel). Strong nuclear staining for NFATc1 in cancer cells was observed in 16 of 23 specimens. NFATc1-positive tumor cells consistently also stained positive for cytoplasmic and nuclear calcineurin B. Scale bars: 50 μm. (D) Expression levels of NFATc1 and calcineurin in pancreatic cancer cell lines as determined by RT–PCR (upper panel) and Western blot (lower panel) analyses. The human RPLP0 gene was used as housekeeping gene control for the human cell lines and the murine cyclophilin A gene (mPPIA) for the murine cell lines in the RT–PCR experiments. The Western blot for NFATc1 shows three major bands corresponding to different phosphorylation states of the protein. CnB=calcineurin B.