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. 2006 Jul 27;25(15):3618–3626. doi: 10.1038/sj.emboj.7601249

Figure 6.

Figure 6

Rescue of hFis1 expression phenotype by MITOL coexpression. (A) Mitochondrial fragmentation by hFis1 expression. HeLa cells expressing FLAG-hFis1 were stained with MitoTracker and anti-FLAG antibody. Bars, 10 μm. (B) Coexpression of MITOL WT, but not CS mutant, blocked hFis1-induced mitochondrial fragmentation. HeLa cells coexpressing Myc-MITOL WT or CS mutant with FLAG-hFis1 were stained with MitoTracker, anti-FLAG and anti-Myc antibodies. Bars, 10 μm. (C) Percentages of cells showing each mitochondrial morphology were calculated from 100 hFis1, hFis1/MITOL WT or hFis1/MITOL CS coexpressing cells. Error bars represent s.d. n=3.