TABLE 2.

Reexamination of 39 discordant specimens with multiple qPCRs

qPCR resulta	omp1 qPCR positive	omp1 qPCR negative	Total
23S rRNA qPCR positive	18	20	38
23S rRNA qPCR negative	1	0	1
Total	19	20	39

^aDNA from vaginal cytobrush specimens had been initially examined by a single Chlamydia 23S rRNA, C. psittaci B577 omp1, and C. pecorum omp1 qPCRs. Thirty-nine discordant specimens identified as Chlamydia positive by one qPCR but as negative by the others or positive in both 23S rRNA and omp1 qPCRs but discordant in Chlamydia species identification were reexamined by reamplification by the respective negative qPCR four or five times. Specimens were assayed multiple times to compensate for the random distribution of single target genes in 5-μl-sample DNA aliquots at the observed low target concentrations (12). Specimens were scored as positive if any single of the multiple qPCRs was positive.