Figure 4.

(A) GR modulates htt aggregation in HEK293 cells. wtGR (50 ng) suppresses aggregation of htt(62)-GFP (200 ng) (first bar), whereas GRΔ108–317 promotes its aggregation (second bar) after dex stimulation. (B) Immunofluorescence of HEK293 cells cotransfected with HA-tagged htt(62) (200 ng) and wtGR (50 ng) shows cytoplasmic aggregates minus dex (a), whereas dex stimulation causes diffuse distribution of htt (b). Cotransfection with GRΔ108–317 increases the formation of nuclear aggregates after dex stimulation (c). HA-tagged htt(62) is displayed in red and DAPI nuclear stain in green. (Magnification: ×100.)